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Molecular Biology

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6-I Bacterial Transformation

6-II DNA Electrophoresis

Key Concepts II

Concept 1: How Do Restriction Enzymes Work?

Concept 2: Cutting DNA with Restriction Enzymes

Concept 3: Gel Electrophoresis

Design of the Experiment II

Exercise 1: Preparing the Gels

Exercise 2: Loading the Gel

Exercise 3: Filling the Wells

Exercise 4: Electrophoresis

Exercise 5: Running the Gel

Exercise 6: Staining and Photographing the DNA

Analysis of Results II

Making a Standard Curve for Hindlll DNA Fragments

Making a Standard Curve

Practice Problem 1

Practice Problem 2

Filling the Wells

Hint: Hold the micropipette just over the well. Be careful not to puncture the bottom of the well with the micropipette!